Microbes From Mum: symbiont transmission in the tropical reef sponge Ianthella basta

Marine sponges are essential for the survival and productivity of coral reefs, and host a distinct community of microorganisms. These microbes can shape sponge phenotype by mediating important developmental stages and their mode of transmission can have profound effects on the adaptability of the sponge. However, the characterisation of each microbial taxa throughout the life cycle of a sponge remains challenging, with many sponge species hosting over 3,000 distinct microbial species. Ianthella basta, an abundant reef sponge in the Indo-Pacific, has recently emerged as a model organism for sponge symbiosis research, harbouring only three dominant symbionts. Here, we successfully spawned Ianthella basta in indoor aquaria, characterised its mode of reproduction, and used 16S rRNA gene amplicon sequencing, fluorescence in situ hybridisation, and transmission electron microscopy to characterise the microbial community throughout its life cycle. We confirmed I. basta as being gonochoric and showed that the three dominant symbionts, that make up more than 90 percent of the microbiome, are vertically transmitted from the mother to the offspring, suggesting an obligate relationship between these microbes and their host.

Identifier
Source https://data.blue-cloud.org/search-details?step=~012F4F28D01834548B31C360B0E0CA0BEFF789E9B7F
Metadata Access https://data.blue-cloud.org/api/collections/F4F28D01834548B31C360B0E0CA0BEFF789E9B7F
Provenance
Instrument Illumina MiSeq; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (146.480W, -19.270S, 147.060E, -18.590N)
Temporal Coverage Begin 2018-08-12T00:00:00Z
Temporal Coverage End 2018-08-15T00:00:00Z