This study aims to enrich nitrifiers classified in genus Nitrospira and investigate the physiological properties. Nitrospira is known as the most dominant nitrifier in some acidic soils. However, cultivated samples to study the physiological characteristics of Nitrospira have not been obtained from soils yet. Therefore, in this study, Nitrospira was enriched by continuous feeding incubation system and the phylogeny was analyzed by amplicon sequencing.