Effects of Echinococcus multilocularis infection on the metabolic composition of mouse urine were assessed. 10 mice were perorally (intragastic gavage) infected with 400 E. multilocularis eggs each. 12 control mice were mock infected. Urine samples were collected 11 weeks post infection. After this incubation period urine samples were analyzed by nuclear magnetic resonance spectroscopy (NMR: 1D 1H).
The names are corresponding to the .zip file in the archive
Mouse_urine-EMultilocularis_infected-and_control _1H-1D-NMR.zip
Folder name group urine volume collected [µL]
10 infected 113
20 infected 97
30 infected 47
40 infected 40
50 infected 76
60 infected 44
70 infected 51
80 infected 134
90 infected 42
100 infected 43
110 control 83
120 control 119
130 control 75
140 control 49
150 control 77
160 control 81
170 control 72
180 control 47
190 control 56
200 control 84
210 control 40
220 control 40
Urine samples were centrifuged at maximum speed for 10 min at 4 °C. 35 µl of each sample supernatant were mixed with 35 µl sodium phosphate buffer (D2O:H2O, v/v, 9:1; pH=7.4; 0.01% trimethylsilylpropanoic acid (TSP); 3 mM sodium-azide), vortexed and centrifuged. 60 µl of each sample were transferred into a 1.7 mm diameter NMR tube. 1H NMR spectroscopy was recorded at 300 K on a Bruker DRX 600MHz NMR spectrometer (Bruker Biospin; Rheinstetten, Germany) at 600.29 MHz. Each spectrum was acquired with 2.73 sec acquisition time for 128 scans (spectral width = 20.2 ppm) with a standard pulse sequence (recycle delay-90°−t1-90°-tm-90°-acquire free induction decay (FID)).
