flipping and desorption energetics of cholesterol in lipid-raft environments

DOI

Cholesterol levels in membranes of cellular organelles varies immensely. For example, cholesterol in the plasma membrane (PM) is 35¿45% of the lipid content , while in the endoplasmic reticulum, the site of cholesterol synthesis, it is only 1¿10%. Further, the distribution of cholesterol across the PM is thought to be asymmetric since its outer leaflet is enriched in saturated lipids. These lipids are preferred by cholesterol due to better packing and water-shileding properties compared to unsaturated lipids. Here we propose to investigate the flipping and desorption/absorption behavior of cholesterol in supported flat membranes that mimic the outer PM using time resolved neutron reflectivity. MD simulations suggest that cholesterol flip-flop in these membranes is in the tens of minutes while in purely unsaturated or saturated membranes it is in the millisecond range.

Identifier
DOI https://doi.org/10.5286/ISIS.E.73940442
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/73940442
Provenance
Creator Dr Ursula Perez-Salas; Dr Yuri Gerelli; Dr Lionel Porcar; Dr Maxmilian Skoda
Publisher ISIS Neutron and Muon Source
Publication Year 2019
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2016-02-25T09:00:00Z
Temporal Coverage End 2016-02-29T09:00:00Z