Integrating organic semiconducting polymers with biological systems is essential for advancing bioelectronic devices. While synthetic modifications and advanced processing techniques are commonly used to enhance cell adhesion and growth, this study introduces UV-Ozone (UVO) treatment as a simple and accessible alternative for modifying pDPP3T films. UVO exposure increases the surface polarity of the semiconductor, as verified by contact angle measurements and XPS analysis. Optimized treatment durations (t ≥ 30 s) significantly improve Schwann cell growth, achieving comparable performance to standard tissue culture plastic (TCP). However, prolonged UVO exposure induces photobleaching, progressively degrading the polymer’s optical and semiconducting properties beyond the optimal threshold. To validate the biointerfacing capability of UVO-treated pDPP3T, electrical impedance spectroscopy was employed to assess cell viability and proliferation on the semiconducting surface. This study highlights UVO treatment as an effective method for integrating conjugated polymers with biological environments, paving the way for further exploration of the interactions between ion transport and semiconductor electroactivity in the context of living cells.