Context and methodology

This data was collected within the framework of the PhD thesis by Antonella Fantoni. The thesis has been published in TU Wien's reposiTUm as referenced below. In this thesis, novel concepts for the preparation of biocompatible photopolymers have been explored. The thesis is divided in two main chapters, beginning with the development of orthogonal photo-crosslinking and -degradation of hybrid poly(vinyl alcohol) and gelatin-based hydrogels. Therein, one-photon radical induced thiol-ene photoconjugation and two-photon radical-induced disulfide scission were facilitated using a simple dithiol-disulfide linker. Parts of this chapter have been published as https://doi.org/10.1021/acs.biomac.5c00062. The second chapter aimed for the synthesis, characterization and application of novel aliphatic radical photoinitiators based on the α-ketoester moiety in vinyl monomers that are known to exhibit less phototoxicity than traditionally used aromatic counterparts. Parts of this chapter have been published as https://doi.org/10.1039/D5MA00926J.

Technical details

File types:

.czi files (Carl Zeiss Image, z-stack LSM images of micropatterned hydrogels) are processable using Zeiss ZEN software or ImageJ with the ImageJ-based Fiji software package.

Bruker NMR raw data directories (pdata, acqu, shimvalues, etc.) are processable with MestreNova or Bruker TopSpin.

More info concerning file types can be found in the General Data Management Plan of the Division Macromolecular Chemistry.

Organisation:

The organization of the data set follows the main chapters of this thesis (“Part A”, “Part B”, “Appendix”) and includes collection of the artwork produced in this thesis.

The PDF files “Labjournal Fantoni 1 & 2” contain a copy of the laboratory notebooks of this thesis.

The .pptx file “Artwork” gathers Figures and Schemes depicted in this thesis.

The zip-file “Part A” contains raw data and meta data of the first chapter of this PhD thesis and contains the following subfolders/Excel files organized by the methodologies used for acquisition. A separate .txt file (“Part A Raw Data_Explanation”) references all substances and abbreviations used in this chapter.

“Part A - 2P Micropatterning_LSM images” is a .zip folder and contains .czi files of the micropatterned hydrogels of day 1 and day 7. The methodology is presented in the Experimental Part A Chapter 4.4 and 5.6 of the related works document

“Part A – Meta data” is a .xlsx file and contains all processed meta data (RT-NIR photorheology, swelling, degradation, 2P micropatterning) presented in this chapter.

“Part A – NMR Raw Data” is a .zip folder containing raw NMR data (1H, 13C) of the macromers and crosslinkers synthesized herein.

“Part A – Photorheology data” is a .xlsx file and contains the raw data of all acquired RT-NIR photorheology measurements of various hydrogels. The methodology is presented in the Experimental Part A Chapter 2.3, 3.2, 4.1 and 5.3 of the related works document.

The zip-file “Part B” contains raw data and meta data of the second chapter of this PhD thesis and contains the following subfolders/Excel files organized by the methodologies used for acquisition. A separate .txt file (“Part B Raw Data_Explanation”) references all substances and abbreviations used in this chapter.

The PDF files “Part B - Elemental Analysis_Part 1 & 2” contain the results of the elemental analysis of the synthesized compunds KE1, KE2, KE3 and KE4, conducted at the Microanalytical Laboratory the University of Vienna.

“Part B – Biocompatibility” is a .xlsx file and contains the raw data of the biocompatibility screening of KE1, KE2, KE3, IC2959 and Li-TPO including plate reader scans and the statistical evaluation using a Dixon’s Q test of the presto blue assay. The methodology is presented in the Experimental Section B Chapter 5 of the related works document.

“Part B – Meta data” is a .xlsx file and contains all processed meta data (UV-Vis, Photo-DSC, RT-NIR photorheology, Biocompatibility, Rheology) presented in this chapter.

“Part B – NMR Raw Data” is a .zip folder containing raw NMR data (1H, 13C) of the compounds synthesized herein.

“Part B – Photo-DSC” is a .xlsx file containing dynamic scanning calorimetry measurements of acrylic, methacrylic and hydrogel formulations containing various photoinitiators (+ co-initiators), irradiated with either 320-500 nm, 365 nm or 358 nm. The methodology is presented in the Experimental Section B Chapter 4.2, 6.1.1 and 6.2.2.1 of the related works document.

“Part B – Photorheology” is a .xlsx file containing RT-NIR photorheology measurements of acrylic, methacrylic and hydrogel formulations containing various photoinitiators (+ co-initiators), irradiated with either 320-500 nm or 358 nm. The methodology is presented in the Experimental Section B Chapter 4.3 and 6.2.2.1 of the related works document.

“Part B – UV-Vis” is a .xlsx file contains the unprocessed absorption and processed extinction data of photoinitiators this chapter. The methodology is presented in the Experimental Section B Chapter 4.1 of the related works document.

“Part B - Raw Files_3D printing” is a .zip folder containing unprocessed images of all 3D printed structures described in the related works document.

“Part B - Raw Files_UV-NIL” is a .zip folder containing SEM images of all structures produced via UV-NIL described in the related works document.

The zip-file “Appendix” contains raw data and meta data of the of the second chapter of this PhD thesis and contains the following subfolders/Excel files organized by the methodologies used for acquisition..

“Appendix - Meta Data” is a .xlsx file and contains all processed meta data (RT-NIR photorheology, degradation, NMR model studies) presented in this chapter.

“Appendix - Raw Data” is a .xlsx file and contains the raw data of the RT-NIR photorheology measurements of hydrogels (related to Part A) and thiol-ene formulations (related to Part B).

"Appendix - NMR study_KE1+Dimercaptopropane" is a .zip folder containing the unprocessed raw NMR data (1H, 13C, HSQC, HMBC) of the model reaction between KE1 and dimercaptopropane.