Submarine canyons are features on the seafloor that support different benthic taxonomic composition and high biodiversity. In the upper Porcupine Bank Canyon, hull mounted multibeam echosounder (EM302) data, Conductivity-Temperature-Depth profiles, current profiles and videos of the seafloor were obtained with an 30 Hz MBES (EM302), SBE 911 CTD, 1Hz Nortek Aquadopp ADCP and the Holland I ROV to understand how seafloor bathymetry, water mass, hydrography and habitat heterogeneity supports the different taxonomic composition and biodiversity in the canyon. Survey measurements were carried out in 2017 and 2018 between 550 m to 1054 m water depth (within three site locations) to determine the spatial distribution of benthic megafauna. Non-coral habitats contained more species, and had differing taxonomic composition.