Data that have been used to create the supplementary material of the article entitled "Synthetic lipopeptides that interact with lipopolysaccharides are potent bactericidal compounds against Xylella fastidiosa" and submitted for review to the journal "Applied and Environmental Microbiology. Data related to with sequences, references, retention time, purity, and mass spectrometry data of peptides; Electrophoresis gel showing extracts of LPS from X. fastidiosa subsp. fastidiosa; Effect of selected peptides at 150 µM on LPS neutralization; LPS neutralization and bactericidal activity of the 36 peptides screened; Dose-response relationship between the reduction in viability of X. fastidiosa subsp. fastidiosa IVIA 5387.2 and peptide concentration after 3-hour contact with selected peptides; Relationship between bactericidal activity of peptides against X. fastidiosa and LPS neutralization; Biological activity of lipopeptides. Bactericidal activity against X. fastidiosa, LPS neutralization, hemolysis and effect on tobacco leaves; Microscopy images after a contact test with CF or BP473-K(CF) and X. fastidiosa; General sequence of lipopeptides with the best biological activity profile studied in this work: BP389 (Phe4, Lys6, Lys10(COC3H7)), BP473 (D-Phe4, Lys6(COC3H7), Tyr10), and BP475 (D-Phe4, Lys6, Lys10(COC3H7)); Hemolytic activity and size of the lesion in infiltrated tobacco leaves; LPS neutralization test of peptides with previously described LPS interaction at 25 µM, using lyophilized E. coli 0111:B4 LPS; LPS neutralization test for the reference peptide YW12D at different concentrations