Seagrass mimics were used to assess the modifications imparted by the mimics on the flow field. For this purpose, laboratory experiments were carried out in September 2023 at the Ludwig-Franzius-Institute of Hydraulic, Estuarine and Coastal Engineering, Leibniz University Hannover, Germany. The seagrass mimics were fabricated from low-density polyethylene (LDPE) with a density of 920 kg/m^3 and a modulus of elasticity of 2.64 ×10^8 N/m^2. Four tropical seagrass species (Enhalus acoroides, Thalassia hemprichii, Halodule uninervis, Halophila ovalis ssp. bullosa) were mimiced by different modelling parameters (see Table 1 in additional metadata file). The full canopy mimic model covered a planform area of 2m × 1m. The seagrass mimic canopy was exposed to unidirectional flow in a recirculating flume with a straight channel of 17.6 m length and 2 m width. The experiments were conducted under four depth-averaged velocities at a constant water depth of 40 cm: 0.13 m/s, 0.21 m/s, 0.28 m/s, 0.35 m/s. Instantaneous velocity measurements were acquired using a stereoscopic Particle Image Velocimetry (PIV) system and a four-beam Acoustic Doppler Velocimeter (ADV). The PIV system measured the three velocity components, namely streamwise, lateral, and vertical velocity components, in a two-dimensional plane in the wake of the canopy centreline (2D3C), while the ADV system measured the same three velocity components at discrete points along vertical profiles at selected streamwise positions relative to the trailing edge of the canopy. The detailed experimental setup, used methods, and data processing are described in the additional metadata file.