This data collection contains dynamic microtomography scans of the tympanic membrane from three fresh-frozen human temporal bones under acoustic stimulation. The data were acquired at the TOMCAT Beamline X02DA of the Swiss Light Source (Paul Scherrer Institute, Villigen, Switzerland). Additionally, we provide spatial transformations across the phases of motion determined via non-rigid registration to quantify tympanic membrane motion.

Three temporal bones (referred to as Fresh1, Fresh7, and Fresh8) were scanned under the following conditions: * Fresh1 (right ear) stimulated at 127 Hz and 126, 141, and 151 dB SPL. * Fresh7 (right ear) stimulated at 128, 256, and 512 Hz and 120 dB SPL. * Fresh8 (left ear) stimulated at 128 Hz and 120 dB SPL.

The microtomography datasets have pixel size of 2.75 um and have been reconstructed in phase contrast for 10 phases of motion. Additionally, non-rigid registration results that allow for calculation of tympanic membrane motion are provided.

Image files are provided in metaimage format and registration parameters are based on the format used by elastix/transformix. Note that the full resolution data (2.75 um pixel size) was given a pixel spacing of 0.25 in the metaimage header information.

For each scan, two datasets are available: * Dynamic microtomography reconstructions over 10 phases covering the tympanic membrane. Additionally, a segmentation of the tympanic membrane and a coarse mask for tympanic membrane registration are provided. The tympanic membrane segmentations from the 4x downsampled data are also provided, which are the basis for meshes used for motion analysis. Where available, 4x downsampled microtomography datasets are provided. * Spatial transformations determined via non-rigid registration to map coordinates from the reference phase to all other phases. Additionally, registration logs are provided, which include a full description of the registration parameters as well as convergence curves. Manually determined landmark pairs are also provided where available for validation.

For the registration files, note that the following reference phases were used: * Fresh1 127 Hz 126 dB - 0 * Fresh1 127 Hz 141 dB - 0 * Fresh1 127 Hz 151 dB - 0 * Fresh7 128 Hz 120 dB - 3 * Fresh7 256 Hz 120 dB - 0 * Fresh7 512 Hz 120 dB - 3 * Fresh8 128 Hz 120 dB - 3

The tomography data are arranged as follows: fresh7_normal_128Hz_120dB - 20.500.11935/9f1f4b1b-2031-4dc9-b170-e29a9d4dc70a fresh7_normal_256Hz_120dB - 20.500.11935/1b6dda1a-97a0-467e-be71-7bffbaabc37a fresh7_normal_512Hz_120dB - 20.500.11935/c0bf6d6a-5f43-4bea-8f3f-a70097603f41 fresh8_normal_128Hz_120dB - 20.500.11935/84bc6621-5a5e-4fc6-b987-431e27f47fce fresh1_normal_127Hz_126dB - 20.500.11935/c59a8c98-2bce-417f-b5bb-a6238b3a5ba3 fresh1_normal_127Hz_141dB - 20.500.11935/93e490bd-fbbb-4505-a978-9d8e901d06e1 fresh1_normal_127Hz_151dB - 20.500.11935/4a571af3-f464-43c1-b33d-3774c0e0bceb

Registration results are arranged as follows: registrations_fresh7_normal_128Hz_120dB - 20.500.11935/46d0a498-d840-4a33-9c63-60b814733524 registrations_fresh7_normal_256Hz_120dB - 20.500.11935/f93ac218-3db3-4864-ac9e-b9ae11e35eca registrations_fresh7_normal_512Hz_120dB - 20.500.11935/ad9f4df4-40a7-4ea9-8336-dc7bf9f8f342 registrations_fresh8_normal_128Hz_120dB - 20.500.11935/8736ac88-e393-4445-9f86-cba766d8f547 registrations_fresh1_normal_127Hz_126dB - 20.500.11935/4a16f953-9376-4113-b443-acfc238f9be5 registrations_fresh1_normal_127Hz_141dB - 20.500.11935/a436dd4a-d445-44f3-bd28-90c166d5ee67 registrations_fresh1_normal_127Hz_151dB - 20.500.11935/9b7e75a6-b4d7-4570-baef-bf9286c1bdee