Raw Data of Activity of Lactobacillus gasseri and its exopolysaccharides in inflammatory cell model and on Candida albicans proliferation.

DOI

These data correspond to the raw data from the article "Activity of Lactobacillus gasseri and its exopolysaccharides in inflammatory cell model and on Candida albicans proliferation". They correspond to the original files for Figures 1, 2, 3, 4, 5 or Figures 1 and 2 Supplementary data from the article, which aimed to observe by confocal microscopy the co-incubation of Candida albicans and Lactobacillus gasseri, each labelled with Concanavalin A-rhodamine and DAPI, respectively; and to verify the purity of the exopolysaccharides obtained, in particular the absence of protein in the exopolysaccharide extractions by SDS-PAGE. The data include the images corresponding to confocal microscopy of Candida albicans, Lactobacillus gasseri or both microorganisms co-incubated for hours to observe their co-incubation, as well as the original images obtained by confocal microscopy or the original images from SDS-PAGE. The data include raw data, calculations and statistics used for the relative expression of pro-inflammatory cytokines (from Caco-2 cells) and inflammatory markers by qPCR, as well as the protein levels secreted by macrophages in the presence of L. gasseri or their EPS by ELISA. The data also include analysis of AhR activity induced by LgEPS. In parallel, MTT analyses highlight the effects of LgEPS on the proliferation of Caco-2 cells. Some of the results obtained on the effects of LgEPS on C. albicans are also presented via biofilm by Crystal Violet and by absorbance measurements of Candida growth in the presence of LgEPS.

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Identifier
DOI https://doi.org/10.57745/GKUZRH
Metadata Access https://entrepot.recherche.data.gouv.fr/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.57745/GKUZRH
Provenance
Creator Charlet, Rogatien ORCID logo; Lecointe, Karine ORCID logo; Plu, Oksana; Cordier, Camille ORCID logo; Bortolus, Clovis (ORCID: 0009-0005-3567-582X); Popczyk, Pierre ORCID logo; Dubar, Faustine ORCID logo; Grec, Sebastien ORCID logo; Sendid, Boualem ORCID logo
Publisher Recherche Data Gouv
Contributor Charlet, Rogatien; Université de Lille; Entrepôt Recherche Data Gouv
Publication Year 2025
Rights etalab 2.0; info:eu-repo/semantics/openAccess; https://spdx.org/licenses/etalab-2.0.html
OpenAccess true
Contact Charlet, Rogatien (Univ. Lille, CNRS, UMR 8576, UGSF - Inserm U1285 - CHU Lille)
Representation
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Version 3.0
Discipline Life Sciences; Medicine