This data set consists of aircraft-based in-situ measurements of different trace gas species during the ACLOUD campaign 2017. Carbon monoxide (CO), carbon dioxide (CO2) and water vapor (H2O) mixing ratios were measured with a time resolution of 1 s. For ozone (O3), the time resolution was lower (2 s) due to the sampling rate of the O3 instrument. There are no CO data available for ACLOUD flight #23 because of an instrumental failure. For ACLOUD flight #14, there are no O3 data available after 12:58:37 UTC.