Replication Data for: Micro Immune Response On-chip (MIRO) models the tumour-stroma interface for immunotherapy testing

Dataset

DOI

Immunotherapies are beneficial for a considerable proportion of cancer patients, but ineffective in others. In vitro modelling of the complex interactions between cancer cells and their microenvironment could provide a path to understanding immune therapy sensitivity and resistance. Here we develop MIRO, a fully humanised in vitro platform to model the spatial organisation of the tumour/stroma interface and its interaction with immune cells. We find that stromal barriers are associated with immune exclusion and protect cancer cells from antibody-dependent cellular cytotoxicity, elicited by targeted therapy. We demonstrate that IL2-driven immunomodulation increases immune cell velocity and spreading to overcome stromal immunosuppression and restores anti-cancer response in refractory tumours. Collectively, our study underscores the translational value of MIRO as a powerful tool for exploring how the spatial organisation of the tumour microenvironment shapes the immune landscape and influences the responses to immunomodulating therapies.

Olympus cellSens Standard, 1.16

Micro-Manager, 2.0

MATLAB, R2021b

ImageJ, 1.54k

CT-FIRE, 3.0 beta

Identifier
DOI	https://doi.org/10.34810/data1891
Related Identifier	IsSupplementedBy https://github.com/xt-prc-lab/Perucca_et_al_2024_Nature_Communications.git
Metadata Access	https://dataverse.csuc.cat/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34810/data1891

Provenance
Creator	Perucca, Alice ; Gomez-Llonin, Andrea ; Mañé Benach, Oriol ; Hallopeau, Clément ; Rivas, Elisa Isabel ; Linares, Jenniffer ; Garrido, Marta; Sallent Aragay, Anna ; Golde, Tom ; Colombelli, Julien ; Dalaka, Eleni ; Linacero, Judith; Cazorla, Marina; Galan, Teresa ; Pastor Viel, Jordi; Badenas, Xavier; Recort-Bascuas, Alba ; Comerma Blesa, Laura ; fernandez nogueira, patricia ; Rovira, Ana ; Roca-Cusachs, Pere (ORCID: 0000-0001-6947-961X); Albanell, Joan ; Trepat, Xavier ; Calon, Alexandre ; Labernadie, Anna
Publisher	CORA.Repositori de Dades de Recerca
Contributor	IBEC, Admin; Institut de Bioenginyeria de Catalunya
Publication Year	2025
Funding Reference	Ministerio de Ciencia, Innovación y Universidades PID2022-142672NB-I00 ; Ministerio de Ciencia, Innovación y Universidades PID2021-128635NB-I00 ; European Research Council AdG-101097753 ; European Research Council Adv-883739 ; European Commission H2020-FETPROACT-01-2016-731957 ; Generalitat de Catalunya 2017-SGR-1602 ; La Caixa Foundation LCF/PR/HR20/52400004
Rights	CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess	true
Contact	IBEC, Admin (IBEC)

Representation
Resource Type	Program source code; Dataset
Format	text/plain; application/pdf; text/csv; image/tiff; application/matlab-mat; text/tab-separated-values; text/plain; charset=UTF-8; image/png; image/jpeg; text/xml
Size	31870; 5645973; 407522; 16044; 64641; 16179; 145952; 21047; 51749; 50036; 45696; 31179; 26519; 30420; 11463; 233444; 186509; 22205; 37843; 186950; 24053; 35330; 24474; 24966; 10454; 4252; 8501; 16555; 38887; 123479; 95394; 116635; 36881; 4478; 27287; 52396; 71096; 46898; 31339; 26768; 29201; 25977; 33732; 28173; 29370; 18195; 22283; 22987; 22707; 4194517; 262357; 1048789; 261457; 520119; 804695; 2219979; 151; 160767; 128867; 514681; 700965; 882; 883; 10418; 716908; 855412; 979; 922; 1682; 2375; 6097; 1701; 938; 2411; 6214; 1964; 1074; 2766; 7123; 935; 1717; 2425; 6232; 281931; 44754; 21270; 126325; 1048724; 23937; 1594705; 3145882; 136827; 33738; 28642; 1315976; 134860; 41705; 12370; 884412; 134698; 17376; 352757; 510669; 1859863; 2109121; 672069; 1312908; 1173132; 1429956; 1373954; 697469; 1224867; 1534543; 34613985; 60746666; 75953; 12677087; 1036358; 15087552; 54484252
Version	1.1
Discipline	Biology; Immunology; Life Sciences; Medicine; Microbiology, Virology and Immunology