This dataset contains the protocols and data from five behaviour tests performed in laying hen pullets, namely a detour test (DT), voluntary approach test (VAT), open field test (OFT), tonic immobility test (TI) and a manual restraint test (MR). These tests were performed as part of a 2x2 factorial design study that investigated the effects of light during incubation and enrichment during rearing on behaviour and physiology of laying hen pullets. This experiment was part of PPILOW. This project has received funding from the European Union’s Horizon 2020 Research and Innovation Programme under grant agreement number 816172.