Raw data from a series of experiments aimed to evaluate the feasibility of producing sophorolipids in solid-state fermentation using trickle-bed reactors.

METHODOLOGICAL INFORMATION

This study conducted solid-state fermentation (SSF) using Starmerella bombicola yeast in a trickle-bed reactor (TBR), with optimized conditions for sophorolipid (SL) production. SL yield was quantified through solvent extractions and analyzed using HPLC, evaluating the effects of glucose concentration, percolation time, and frequency

1. Description of methods used for collection-generation of data: Lab scale experiments were carried out using 5 0.5-L reactors.

2. Methods for processing the data:

3. SLs-crude and remaining oil were analytically determined after solvents extraction using ethyl acetate and n-hexane.
4. SLs concentration concentration was quantified by HPLC-UV.

5. Glucose was quantified by YSI equipment.

6. Instrument- or software- specific information needed to interpret the data:

7. Excel or similar software.

8. Instruments, calibration and standards information:

9. Analytical balance Sartorius Entris II
10. Orbital shaker (Infors HT, UK)
11. HPLC UltiMateTM 3000

12. Macherey-Nagel™ Nucleosil™ 100 mm × 3 μm x 4.7 mm C18 EC column

13. Environmental or experimental conditions:

14. Starmarella bombicola (CECT 11854) was cryopreserved at -80°C.
15. Microorganism growing conditions were set up at 30 ºC, 180 rpm at 48 h.

16. 0.5-L bioreactors operational parameters were established at: temperature 30 ºC, humidefied airflow rate 30 mL/min.

17. Quality-assurance procedures performed on the data: All time course experiments were carried out using 8 reactors, sacrificing 2 each 2 day.