To investigate the variability of the Atlantic Meridional Overturning Circulation (AMOC) during the Holocene in the North Atlantic, we analyzed radioisotopes (230Th, 231Pa, 232Th, 234U, and 238U) in sediment samples from four core sites (ODP 172-1063, ODP 172-1059, ODP 162-983, and GeoB18529-2) with millennial-scale temporal resolution. From these measurements, we calculated 231Pa/230Th ratios. The isotope analyses were conducted using three instruments: a Neptune Plus MC-ICP-MS equipped with a retarding potential quadrupole filter at the Geozentrum Nordbayern, Friedrich–Alexander University, Erlangen; an ELEMENT ICP-MS at the Alfred Wegener Institute (AWI), Bremerhaven; and an iCAP TQe ICP-MS at the Institute of Earth Sciences, Heidelberg University.To assess the influence of biogenic opal (bOpal) concentrations on the 231Pa/230Th ratios, we measured bOpal concentrations from the same samples. These measurements followed an automated protocol for dissolved silica analysis using molybdate-blue spectrophotometry, conducted at Heidelberg University.The dataset includes estimates of mean sub-millennial AMOC strength in the North Atlantic, derived from the combined sedimentary 231Pa/230Th data. Using AMOC- 231Pa/230Th relationships from the Bern3D Earth system model, we converted the 231Pa/230Th ratios from all cores into a unified time series of past AMOC strength (expressed in Sverdrups, Sv) and applied a generalized additive model (GAM) fit for trend analysis.