Here, two-photon polymerization direct laser writing is presented as a method to add dynamic functionality to continuous-phase microfluidic chips by integrating thermoresponsive poly(N-isopropylacrylamide) (pNIPAM) microactuators. Exploiting the polymer’s lower critical solution temperature and tailored designs, efficient catch-and-release of polystyrene beads and filtering of (single)-cells by size are demonstrated. This approach enables flexible microfluidic devices with enhanced functionality.

This dataset accompanies the manuscript "Two‐Photon Direct Laser Writing of pNIPAM Actuators in Microchannels for Dynamic Microfluidics". It includes microscopy images (*.tiff), video clips and confocal z-stacks.