Field investigations were performed in four areas: a treeless mountainous tundra (16-KP-04; Lake Rauchuagytgyn area), tundra-taiga transition zone (16-KP-01, Lake Ilirney area) and a northern taiga (18-BIL-01, 18-BIL-02). In total, 39 sites were investigated. The sites were placed to cover different vegetation communities that characterise central Chukotka. Fifteen-meter radius sample plots (sites) were demarcated in the most homogeneous locations. Heterogeneity was accommodated by roughly assorting vegetation into two to three vegetation types per sampling plot. Within each area of roughly estimated vegetation types we selected one 0.5 x 0.5 m subplot for representative ground-layer above-ground biomass (ABG) harvesting (major taxa and other). For moss and lichen AGB harvesting inside 0.5 x 0.5 m subplots representative 0.1 x 0.1 m subplots were chosen. All harvested AGB samples were weighed fresh in the field. In general, AGB samples with a weight of more than 15 g were subsampled. All samples were oven dried (60 °C, 24 h for ground-layer and moss and lichen samples, 48 h for shrub and tree branch samples) and weighed again. This dataset contains the raw data of dry weight for each sub-ground vegetation type sampling plot. All data was collected by scientists from Alfred Wegener Institute (AWI), Helmholtz Centre for Polar and Marine Research and University of Potsdam, Germany, The Institute for Biological problems of the Cryolithozone, Russian Academy of Sciences, Siberian branch, and The Institute of Natural Sciences, North-Eastern Federal University of Yakutsk, Yakutsk, Russia.The AGB data calculations for the plot area including tree and tall shrubs can be found at https://doi.org/10.1594/PANGAEA.923719.