Crowding in ternary protein solutions

DOI

The interior of living cells is occupied by macromolecules such as proteins, which occur at a high volume fraction on the order of 30% in the aqueous solution of the cellular fluid. The issue of macromolecular crowding is therefore of primordial importance for the function of living cells [R.J. Ellis, Curr. Opin. Struct. Biol. 2001, 11, 114]. To model the situation of crowding in vitro, we propose to investigate the diffusion of protein(BSA)-protein(IgG)-water(D2O) ternary mixtures, where the proteins BSA (bovine serum albumin) and IgG (immunoglobulin) are important constituents of blood. Using our previous large QENS data sets of BSA-water and IgG-water binary mixtures and consistent modeling, we expect to be able to separate the contributions of the two proteins to the QENS spectra in the ternary mixture.

Identifier
DOI https://doi.org/10.5291/ILL-DATA.9-13-526
Metadata Access https://data.ill.fr/openaire/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=10.5291/ILL-DATA.9-13-526
Provenance
Creator Zhang, Fajun; Roosen-Runge, Felix; Schreiber, Frank; Grimaldo, Marco; Feustel, Michal; Da Vela, Stefano; Seydel, Tilo
Publisher Institut Laue-Langevin
Publication Year 2014
Rights OpenAccess; info:eu-repo/semantics/openAccess
OpenAccess true
Representation
Resource Type Dataset
Size 19 GB
Version 1
Discipline Particles, Nuclei and Fields