The file contains the CTCF-DNA binding sites for complete genome of Homo sapiens (human), Mus musculus (mouse), Sus scrofa (pig), Capra hircus (goat), Aedes aegypti (dengue and yellow fever mosquito) and Drosophila melanogaster (fruit fly) using electronic nucleotide alignment. The data analysis is described in NN Oiwa, K Li, CE Cordeiro and DW Heermann, 2022 [arXiv:2110.10508]. Detailed description of the Electronic Nucleotide Alignment method can be found in NN Oiwa, CE Cordeiro, DW Heerman (2016). We also add Caenorhabditis elegans (worm), Plasmodium falciparum (protozoan) and Arabidopsis thaliana (plant) as negative test, since there is not CTCF in these species.