Supplementary material for 'Structural basis for ninjurin-1 mediated plasma membrane rupture in lytic cell death'

DOI

Eukaryotic cells can undergo different forms of programmed cell death, many of which culminate in plasma membrane rupture (PMR) as the defining terminal event. PMR was long thought to be driven by osmotic pressure, until it was recently shown to be in many cases an active process, mediated by the protein ninjurin-1 (NINJ1). Here, we studied whether the newly resolved cryo-electron microscopy NINJ1 filaments are capable to cap membrane edges by multiscaling molecular dynamics simulations. Site-directed mutagenesis was employed to validate the function of the resulting supramolecular arrangement. The membrane protein NINJ1 is thus an interactive component of the eukaryotic cell membrane that functions as an in-built breaking point in response to activation of cell death.

GROMACS, 2020 and 2021

Identifier
DOI https://doi.org/10.18419/darus-3373
Related Identifier IsCitedBy https://doi.org/10.1038/s41586-023-05991-z
Metadata Access https://darus.uni-stuttgart.de/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.18419/darus-3373
Provenance
Creator Degen, Morris ORCID logo; Santos, José Carlos ORCID logo; Pluhackova, Kristyna ORCID logo; Cebrero, Gonzalo ORCID logo; Ramos, Saray ORCID logo; Jankevicius, Gytis ORCID logo; Hartenian, Ella ORCID logo; Guillerm, Undina; Mari, Stefania A. ORCID logo; Kohl, Bastian ORCID logo; Müller, Daniel J. ORCID logo; Schanda, Paul ORCID logo; Maier, Timm ORCID logo; Perez, Camilo ORCID logo; Sieben, Christian ORCID logo; Broz, Petr ORCID logo; Hiller, Sebastian ORCID logo
Publisher DaRUS
Contributor Pluhackova, Kristyna
Publication Year 2023
Funding Reference DFG EXC 2075 - 390740016 ; Ministry of Science, Research and the Arts Baden-Württemberg HoreKa supercomputer ; Federal Ministry of Education and Research HoreKa supercomputer
Rights CC BY 4.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/licenses/by/4.0
OpenAccess true
Contact Pluhackova, Kristyna (University of Stuttgart)
Representation
Resource Type Files and structures for molecular dynamics simulations; Dataset
Format application/x-gzip
Size 47015759; 32445420; 399837; 5028835; 4617408; 24363844; 14976519; 5215291; 2397772; 1724476; 7052321
Version 1.1
Discipline Life Sciences; Medicine