We have studied the effects of slow infiltration of oxygen on microbial communities in refrigerated legacy samples from ocean drilling expeditions. Storage was in heat-sealed, laminated foil bags with a N2 headspace for geomicrobiological studies. Analysis of microbial lipids suggests that Bacteria were barely detectable in situ but increased remarkably during storage. Detailed molecular examination of a methane-rich sediment horizon showed that refrigeration triggered selective growth of ANME-2 archaea and a drastic change in the bacterial community. Subsequent enrichment targeting methanogens yielded exclusively methylotrophs, which were probably selected for by high sulfate levels caused by oxidation of reduced sulfur species. We provide recommendations for sample storage in future ocean drilling expeditions.

Supplement to: Lin, Yu-Shih; Biddle, Jennifer F; Lipp, Julius S; Orcutt, B; Holler, Thomas; Teske, Andreas P; Hinrichs, Kai-Uwe (2010): Effect of storage conditions on archaeal and bacterial communities in subsurface marine sediments. Geomicrobiology Journal, 27, 261-272