Benthic macrofauna were sampled on expedition HE625 of the R/V Heincke in July 2023 at 50 stations spread evenly over the muddy sediment region in southeast of the island of Helgoland (southeastern North Sea). At each station, two replicate samples were taken with a van Veen grab with a sampling area of 0.1 m² and a penetration depth of 10 cm. The first sample was sieved through a 1000 µm mesh to retain macrofauna, and species were determined to the lowest taxonomic level. The WoRMS LSIDs (AphiaID) was provided to identify the scientific name of the species. For each taxon, the total wet weight was measured with a precision of 0.001 g. The dataset comprises counted abundance and biomass records for 171 infaunal taxa.