This dataset documents seasonal heavy metal bioaccumulation in two commercially important edible freshwater fish species, Labeo rohita (Rohu) and Cirrhinus mrigala (Mrigal carp), collected from Head Panjnad, Punjab, Pakistan (29.3464 °N, 71.0197 °E), during summer and winter seasons of 2022. Head Panjnad represents the confluence zone of the major Punjab rivers and forms an important hydrological transition between the upper and lower Indus Basin. Fish specimens were collected from multiple locations near the Panjnad headworks.