We obtained the credible target sequences by filtrating high-throughput profiling of muscle tissue in large yellow croaker.The goal of this study is to measured almost all small RNA in muscle not only at the common feeding but also during the fasting period. We set the data of microRNA as a mainspring of study. By bioinformatics analysis, all possible target binding site were compared and predicted in both known and unknown. Overall design: Examination of 2 different starvation periods of large yellow croaker, including one group with normal diet and the other group with fasting by 21 days.