Prokaryotic cell abundance and biomass measured during Maria S. Merian cruise MSM29 and Polarstern cruise PS85 to the Fram Strait


Prokaryotic cell abundances and biomass were determined in deep-sea sediment samples collected at the deep-sea LTER HAUSGARTEN, located in the Fram Strait. Sampling was performed during two cruises, i.e. RV Maria S. Merian MSM 29 in summer 2013 (23 June-12 July) and RV Polarstern PS85 in summer 2014 (6 June-3 July). Undisturbed sediment cores were collected with a TV-guided multicorer. Prokaryotic cell abundances were determined using acridine orange direct counts, following the procedure described in (Hoffmann et al., 2017; doi:10.3389/fmicb.2017.00266). For each sample two replicate filters were counted. Cell volumes were determined using a stage micrometer and converted to biomass using a conversion factor of 3 x 10^-13 g C /µm³ (Børsheim, K. Y., Bratbak, G., and Heldal, M. (1990). Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy. Applied and Environmental Microbiology 56, 352-356).

Related Identifier
Metadata Access
Creator Bienhold, Christina
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2019
Funding Reference Seventh Framework Programme, 294757
Rights Creative Commons Attribution 4.0 International;
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 76 data points
Discipline Earth System Research
Spatial Coverage (-5.290W, 78.506S, 7.003E, 79.758N); North Greenland Sea
Temporal Coverage Begin 2013-06-26T01:11:00Z
Temporal Coverage End 2014-06-25T16:59:00Z