Previous studies have revealed a suites of physiological and molecular adaptations enabled the Antarctic radiation, such as the acquisition of antifreeze proteins and attainment of neutral buoyancy. However, genome-wide characterization of genetic mechanisms underpinning the remarkable radiation is far from adequate. Here we De novo sequenced the genome of the Antarctic toothfish Dissostichus mawsoni and the genome of Patagonia blennie Eleginops maclovinus to represent the high-altitude Antarctic species and the temperate/primitive notothenioid respectively.