Epi-fluorescence micrographs and simulated particle concentration fields used to generate the results shown in Fig.1, Fig. 2, Fig. 3, Fig. 5 and Fig. 6 of "Surface Chemistry-based Continuous Separation of Colloidal Particles via Diffusiophoresis and Diffusioosmosis " by A. Chakra, C. Puijk, G. T. Vladisavljevic, C. Cottin-Bizonne, C. Pirat and G. Bolognesi.Full description of the dataset is provided in the Readme.pdf file.