The latest Cenomanian to early Turonian sedimentary record recovered at IODP Site 369-U1513 in the Mentelle Basin (SE Indian Ocean, paleolatitude about 60°S at 85 Ma) is studied to interpret the paleoceanographic evolution across the Oceanic Anoxic Event 2 (OAE 2) in the Southern Hemisphere. Here we present the planktonic and benthic foraminifera, radiolaria and calcispheres distribution and absolute abundances, the calcareous nannofossils distribution, the CaCO3 content, the bulk carbonate carbon and oxygen stable isotope data and the Total Organic Carbon values from IODP Holes 369-U1513A and 369-U1513D. The data presented here permit interpretation of the dynamics of the water mass stratification and document a reduced water mass stratification with alternating episodes of enhanced surface water productivity and variations of the thickness of the mixed layer across the OAE 2.