The data published here are datasets used in the publication by Oliveira and Feord et al. (submitted), entitled: Ionome characterisation from algae isolated from the terrestrial cryosphere using single cell ICP-ToF-MS. This publication presents data characterising the ionomes of algae isolated from the terrestrial cryosphere (snow and ice surfaces) using single cell ICP-ToF-MS. The publication compares the ionomes of cryopshere algae to mesophilic algae. The single cell ICP-ToF-MS data is compared to traditional ICP-MS data with culture lysates. Cell biovolume is calculated using FlowCam pictures and imageJ measurements of width and length. We also include in this data publication the canonical scores of the discriminant analysis comparing the different algal taxa.

All data originate from algal cultures grown at the GFZ in a laboratory incubator and does not represent any environmental data. Five algal cultures were used in this study: two polar algae species isolated from snow in Svalbard Microglena sp. (CCCryo 002b-99), Raphidonema Sempervirens (CCCryo 011a-99), one polar algae species isolated from the ice Deuterostichococus sp. GUPI-18 (isolated from the Greenland Ice Sheet) and two mesophilic chlorophyte model strains Chlamydomonas reinhardtii (SAG 33.89) and Acutodesmus Obliquus (SAG 276-1).

Five datasets are presented in this data publication:

1. Raw single-cell ICP-ToF-ms data (data used in all figures in Oliveira and Feord et al.

2. Processed single-cell ICP-ToF-MS data (data used in all figures in Oliveira and Feord et al.)

3. Raw ICP-MS data (bulk digestions, data used to calculate single cells/bulk digestion ratios from Fig. 6 in Oliveira and Feord et al.)

4. Processed ICP-MS data (bulk digestions) and associated cell counts (data used to calculate single cells/bulk digestion ratios from Fig. 6 in Oliveira and Feord et al.)

5. Cell length and width data measured from FlowCam images with ImageJ (data used to calculate Carbon:element ratios in Fig. 4 in Oliveira and Feord et al.)

6. Results of the discriminant analysis (data from Fig. 2 in Oliveira and Feord et al.)

All information regarding the sampling methods, analytic procedures, and data processing is available in Oliveira and Feord et al. (submitted).