Archaeorhizomycetes is a class of globally widespread soil-dwelling fungi, originally proposed to be associated with plant roots, but their ecology and nutritional mode are not clearly defined. To increase the knowledge about Archaeorhizomycetes’ ecology and biogeography, we investigate how they are distributed along major environmental gradients, as well as different soil compartments. The dataset consists of mapping files for raw sequence data deposited to ENA under accession numbers ERR15529369- ERR15529373 for PacBio long-read amplicon sequences of Archaeorhizomycetes (partial 18S, ITS, partial 28S, approx. 2500 bp) and accession numbers ERR15529374- ERR15529378 for raw sequencing files for V4 of the 18S using general eukaryotic primers (TAReuk454FWD1 and TAReukREV3). The dataset also consists of OTU tables for both sequencing runs, metadata and R scripts to analyse the data.