The genetic profile of the barbel chub species resembles that of the grass carp. However, barbel chub demonstrates resistance to GCRV, and there remains a dearth of knowledge regarding the immune mechanisms employed by the barbel chub in response to GCRV. In this study, we performed a global transcriptional profiling from four tissues (liver, spleen, head-kidney and trunk-kidney) analysis of GCRV-induced barbel chub to explore the distinctive response mechanism of barbel chub towards GCRV.