The dataset contains the data and the MATLAB scripts associated with the publication titled "Delivery of intraflagellar transport proteins to the ciliary base and assembly into trains ". The data and codes are orgainized in separate folders, with the titles indicating the figure the data belongs to. In addition, there are a couple of folders containing essential scripts used for analysis of single-molecule tracks and numerical simulations performed in the study. Each folder contains a ReadMe.txt file which details the data (and structure) and the accompanying script(s). Data is stored as .csv files and scripts are written in MATLAB (The Math Works, Inc., R2020a).