Evaluating serail crystallography approaches for diamond anvil cell data collection.

DOI

High pressure macromolecular crystallography (HPMX) is an ideal technique to trap high-energy transient conformers of proteins that are essential for biological activity and/or for interaction with partners. Using a diamond anvil cell (DAC) and collecting data at room temperature (RT) ensures that the pressure/temperature of the sample environment is precisely defined and controlled. For proteins, this usually requires large enough crystals to collect a complete data set at high resolution, hopefully radiation damage free. However, growing such crystals remain a challenge. Serial crystallography offers the possibility to collect diffraction data from micron-sized crystals at RT. Indeed, only a single diffraction frame is recorded from a small single crystal, therefore spreading the required X-ray dose to reconstruct a complete dataset over a very large number of micron-sized crystals. we want to evaluate the feasibility to combine SX and DAC techniques to push forward HPMX.

Identifier
DOI https://doi.org/10.15151/ESRF-ES-2116854002
Metadata Access https://icatplus.esrf.fr/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatplus.esrf.fr:inv/2116854002
Provenance
Creator Anne-Claire DHAUSSY; Mohamed MEZOUAR; Nicolas COQUELLE; Max GERIN; Eric GIRARD ORCID logo
Publisher ESRF (European Synchrotron Radiation Facility)
Publication Year 2028
Rights CC-BY-4.0; https://creativecommons.org/licenses/by/4.0
OpenAccess true
Representation
Resource Type Data from large facility measurement; Collection
Discipline Particles, Nuclei and Fields