This dataset includes data collected during a multigenerational crude oil exposure experiment involving Atlantic cod (Gadus morhua) broodstock. Mature cod in late gametogenesis were exposed for 20 days to either a crude oil water-soluble fraction or untreated seawater. After exposure, fish from both groups were repeatedly strip-spawned, and egg batches were fertilized in vitro using pooled sperm from control males. The embryos were then raised in clean seawater until hatching. The dataset encompasses various types of data: housing conditions (temperature and oxygen saturation), mortality, and morphometric measurements of the parental generation. Additionally, it includes water chemistry analysis (polycyclic aromatic hydrocarbons and total extractable organic materials), as well as body burden analysis (polycyclic aromatic hydrocarbons and other crude oil-related organic compounds) in the eggs. Sperm quality parameters and embryo data are also provided, including raising temperature, egg diameter, hatching success, morphology, axial deformation, and cardiotoxicity. This research was conducted at the Tromsø Aquaculture Research Station (Havbruksstasjonen i Tromsø AS, Kårvik) and the Centre for Marine Aquaculture (CAM, NOFIMA AS, Kraknes) and was approved by the Norwegian Animal Research Authority (ID 22461).