The dataset includes the physicochemical and in vivo characterization data supporting the article “Subcutaneous administration of an endocrine-mimetic platform allows for prolonged tumor-uptake of a tumor-targeted protein.” It contains measurements of protein size, polydispersity, zeta potential, morphology, and release kinetics of zinc-induced secretory granules (SGs) obtained by DLS, FESEM, and TEM, as well as fluorescence-based biodistribution data in mice after subcutaneous, intramuscular, and intraperitoneal administration. The results demonstrate that subcutaneous administration ensures the most sustained protein release and highest tumor accumulation of the CXCR4-targeted T22-GFP-H6 protein.

METHODOLOGICAL INFORMATION

1. Description of methods used for collection-generation of data: Data were obtained through the design, production, and characterization of the recombinant protein T22?GFP?H6. The protein was expressed in E. coli after IPTG induction and purified by affinity chromatography. Its purity, integrity, and concentration were analyzed by SDS?PAGE, western blot, MALDI?TOF mass spectrometry, and Bradford assay. Secretory granules were formed by zinc?induced precipitation and characterized by dynamic light scattering (DLS), electrophoretic light scattering (ELS), and electron microscopy (FESEM and TEM). Protein release was measured by UV spectrophotometry. In vivo biodistribution was assessed in a CXCR4? colorectal cancer mouse model using IVIS fluorescence imaging, while tissue analysis was performed by hematoxylin?eosin and immunohistochemistry.

2. Methods for processing the data: GraphPad Prism 8.0.2 software was used to establish statistical significance. An initial assessment of normality and log-normality using the Shapiro-Wilk test was performed to verify the data’s normal distribution. For parametric data, a one-way ANOVA test was applied; for nonparametric  data, the Kruskal-Wallis test was used. All measurements were performed at least in triplicate, and data were expressed as mean ± standard  error of the mean (SEM). Statistical significance (*) was considered to be achieved when p < 0.05. The 3D protein structure.

3. Instrument- or software- specific information needed to interpret the data: GraphPad Prism 8.0.2 software was used to establish statistical significance. An initial assessment of normality and log-normality using the Shapiro-Wilk test was performed to verify the data’s normal distribution. For parametric data, a one-way ANOVA test was applied; for nonparametric  data, the Kruskal-Wallis test was used. All measurements were performed at least in triplicate, and data were expressed as mean ± standard  error of the mean (SEM). Statistical significance (*) was considered to be achieved when p < 0.05. The 3D protein structure.