The reproductive success of frogs depends on their ability to locate, detect and recognize the calls of their own species. However, frogs have difficulties determining the location or origin of sound when the wavelength is larger than the distance between their two ears. Some species have developed morpho-functional adaptations, such as asymmetric auditory canals or physical coupling between the two tympanic membranes, to accentuate the temporal and amplitude differences between their two ears to locate prey in flight. The aim of this study is to investigate the functional morphology of the frog ear in vivo using a high-quality, high-speed X-ray dataset, using an image configuration based on modulation without phase contrast. The experiment will extract the vibratory behavior of the middle ear in 3D using sparse-view CT reconstruction algorithms.