This BAG proposal aims to advance SX studies at ID29. We will investigate the structural dynamics and reaction mechanisms of enzymes and materials relevant to human health and biocatalysis. Our studies focus on AnmK, which catalyzes peptidoglycan recycling in P. aeruginosa; human Fascin1, an actin-bundling protein linked to cancer metastasis; NQO1, a flavoenzyme involved in antioxidant defense; and FNR/FPR, key electron transfer proteins with distinct NADP+/H binding properties. Additionally, we will study FFTR enzymes and purine-metabolizing IMPDH/GMPR to understand their regulatory mechanisms. Beyond enzymes, we propose pump-probe TR experiments on MOFs to capture structural transitions during catalysis and activation. Crystallization and preliminary diffraction tests were performed at ID29, confirming sample quality. Using ID29’s diverse sample delivery methods, we will employ SSX and TR-SX techniques to capture reaction intermediates and conformational transitions in these systems.