Replication Data for: Single cell migration along and against confined haptotactic gradients

DOI

Data generated at the published investigation: Haptotaxis is the process of directed cell migration along gradients of extracellular matrix density and is central to morphogenesis, immune response and cancer invasion. It is commonly assumed that cells respond to these gradients by migrating directionally towards the regions of highest ligand density. In contrast with this view, here we show that cells exposed to micropatterned fibronectin gradients exhibit a wide range of complex trajectories, including directed haptotactic migration up the gradient but also linear oscillations and circles with extended periods of migration down the gradient. To explain this behaviour, we developed a biophysical model of haptotactic cell migration based on a coarse-grained molecular clutch model coupled to persistent stochastic polarity dynamics. While initial haptotactic migration is explained by the differential friction at the front and back of the cell, the observed complex trajectories over longer time scales arise from the interplay between differential friction, persistence, and physical confinement. Overall, our study reveals that confinement and persistence modulate the ability of cells to sense and respond to haptotactic cues and provides a framework to understand how cells navigate complex environments.

Identifier
DOI https://doi.org/10.34810/data2489
Metadata Access https://dataverse.csuc.cat/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34810/data2489
Provenance
Creator Trepat, Xavier ORCID logo; Fortunato, Isabela Corina ORCID logo; Brückner, David ORCID logo; Grosser, Steffen ORCID logo; Nautiyal, Rohit ORCID logo; Rossetti, Leone ORCID logo; Bosch-Padrós, Miquel ORCID logo; Trebicka, Jonel ORCID logo; Roca-Cusachs, Pere (ORCID: 0000-0001-6947-961X); Sunyer, Raimon ORCID logo; Hannezo, Edouard ORCID logo
Publisher CORA.Repositori de Dades de Recerca
Contributor Trepat, Xavier; Institut de Bioenginyeria de Catalunya
Publication Year 2025
Rights CC0 1.0; info:eu-repo/semantics/openAccess; http://creativecommons.org/publicdomain/zero/1.0
OpenAccess true
Contact Trepat, Xavier (Institute for Bioengineering of Catalonia)
Representation
Resource Type Experimental data; Dataset
Format text/plain; text/csv; text/tab-separated-values
Size 26119; 12094; 34012; 276966; 241477; 45; 4235; 389; 394; 3639; 3679; 84335; 91725; 80; 5433; 1526; 1554; 1411; 325; 9658; 1928; 101853; 703; 179494; 26303; 144896; 44; 8466; 1097; 626; 37; 346642; 36; 4227; 129221; 742103; 166015; 859651; 7194; 7236; 54758; 53594; 16232; 33386; 373374; 1077; 245; 2488; 40522; 6011; 82051; 56631; 10117; 30865; 11869; 33; 13267; 64575; 48009; 485; 243692; 134; 49
Version 2.0
Discipline Life Sciences; Medicine; Natural Sciences; Physics