The stratum corneum (SC) as the main penetration barrier of the mammalian skin is very important for ter¬rest¬ri¬al life[1]. The barrier properties are dependent on the SC lipid matrix, which is com¬po¬sed of ceramides (CER), cholesterol and free fatty acids. Un¬fort¬unately their mol¬ec¬u¬lar arrangement is still not completely understood. This led to the development of sev-er¬al different theoretical models, trying to explain SC properties, which are still under de¬ba-te[2]. The aim of this study is to yield more information that will help to fur¬ther sup¬port one of these mo¬dels. Special focus shall be set to the two most recently pro¬po¬sed models, the ar¬ma-tu¬re reinforcement model[3, 4] and the asymmetry model[5]. For both models the full extended conformation of CER is indispensable[3, 4, 5]. That for the asym¬me¬tric CER[NP]-C24 and CER[AP]-C24 shall be in¬ves¬tigated for their influence on the lipid ma¬trix ar¬chi¬tec¬ture, using neutron diffraction and for the first time defined deuterations within these CER molecules.