Towards 3D structure determination and dynamics of biological and non-biological samples using TR-SSX methods

DOI

This BAG proposal aims to conduct room temperature time-resolved serial crystallography experiments at the new ID29 beamline at ESRF. We intend to collect full data sets on microcrystals of two medically relevant proteins, the penicillin binding protein 2a (PBP2a) of methicillin resistant staphyloccocus aureus (MRSA) and the human the NAP(P)H:quinone oxidoreductase 1 (NQO1) protein, with their respective ligands. As well, we aim at conducting pump-probe time-resolved crystallography experiments on microcrystals of metal organic frameworks (MOFs). The good quality of all crystalline samples has been proved either at ESRF beamlines ID30-A1 and ID30-A3, or at other synchrotron beamlines. At ID29, they offer a variety of sample delivery devices including high viscosity injectors, tape drives, fixed target supports, and microfluidic devices, that will be employed in our experiments depending on sample needs.

Identifier
DOI https://doi.org/10.15151/ESRF-ES-1186982707
Metadata Access https://icatplus.esrf.fr/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatplus.esrf.fr:inv/1186982707
Provenance
Creator María BUÑUEL ESCUDERO; Alice GRIECO; Marta MARTINEZ-JULVEZ ORCID logo; Julien ORLANS; JOSE MANUEL MARTIN GARCIA ORCID logo; Alexandra ROS ORCID logo
Publisher ESRF (European Synchrotron Radiation Facility)
Publication Year 2026
Rights CC-BY-4.0; https://creativecommons.org/licenses/by/4.0
OpenAccess true
Representation
Resource Type Data from large facility measurement; Collection
Discipline Particles, Nuclei and Fields