Structure of myoglobin-sugar-water systems

DOI

This proposal is a continuation of our previous experiment on NIMROD (Rb 1520094), where we studied aqueous solutions of trehalose (33 wt% trehalose) as well as myoglobin in the same solution (25 wt% myoglobin). The results of these previous measurements are very interesting and show that water and trehalose are homogenously distributed with plenty of hydorogen bonding inbetween. For the tenary system it is evident that myoglobin is prefentially surrounded by wate and that trehalose in the remaining solution causes a remarkable structural ordering of the myoglobin molecules. The aim of this proposal is both to elucidate whether the results are unique for trehalose, or will be similar for another sugar, and to determine how the ordering of the myoglobin molecules, as well as the distibution of the sugar molecules, are affected by a lower protein concentration and/or another sugar.

Identifier
DOI https://doi.org/10.5286/ISIS.E.87773342
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/87773342
Provenance
Creator Mr Christoffer Olsson; Professor Jan Swenson; Dr Tristan Youngs
Publisher ISIS Neutron and Muon Source
Publication Year 2021
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2017-10-12T08:00:00Z
Temporal Coverage End 2018-02-12T08:58:41Z