Conformational changes of human growth hormone driven by the interaction with non-ionic surfactants

DOI

The tale of protein-surfactant interactions has been the goal of many scientific investigations in the last few decades. The interaction between proteins and amphiphiles in solution are of wide interest due to the ubiquity of these compounds in pharmaceutical formulations and health care products. However, detailed structural models of protein-surfactant complexes are still rather scarce and general consensus is yet missing. The aim of this experiment is to elucidate the mechanism of interaction of human growth hormone with a set of non-ionic surfactants (polysorbate 20, octaethylene glycol monododecyl ether, and dodecylmaltoside) by means of contrast-variation SANS. The main goal will be to identify the characteristics of the surfactant-protein complexes, the effect of the surfactant in the protein conformation and the interactions occurring in each system.

Identifier
DOI https://doi.org/10.5286/ISIS.E.RB1910529-1
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/105598012
Provenance
Creator Dr Judith Houston; Dr Adrian Sanchez-Fernandez; Dr Sarah Rogers; Dr Marie Wahlgren; Dr Helen Sjogren; Mr Johan Larsson
Publisher ISIS Neutron and Muon Source
Publication Year 2022
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2019-09-14T15:03:51Z
Temporal Coverage End 2019-09-17T08:00:00Z