This dataset contains the infrared spectra of CTX-TNA2 rat astrocytes (healthy) and F98 rat glioma (tumour) cell lines subjected to standard proton radiotherapy and proton minibeam radiation therapy (pMBRT), measured via synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-FTIRM).

1. Description of methods used for collection-generation of data: CTX-TNA2 rat astrocytes (healthy) and F98 rat glioma (tumour) cell lines, previously subjected to standard proton radiotherapy or proton minibeam radiation therapy (Centre de Protonthérapie d'Orsay, Institut Curie), were fixated on infrared transparent calcium fluoride coverglasses, suitable for infrared microspectroscopy measurements. Fixation occurred one day post-treatment, and the protocol is detailed in [Martínez-Rovira, et al. (2019). Analyst, 144, 6352]. The SR-FTIRM measurements were conducted at the MIRAS beamline of ALBA Synchrotron (Cerdanyola del Vallès, Spain). Over 100 cells were randomly selected for each sample and irradiation configuration (control, broad beam, minibeam peak, minibeam valley). Single-point maps of each cell were collected in the 3800-900 cm-1 mid-infrared spectral range, using a spectral resolution of 4 cm-1 and 128 co-added scans per spectrum. 2. Methods for processing the data: Spectral data was cut into two distinct spectral ranges: the Higher Wavenumber (HW, 3000-2800 cm-1) or Amides and Fingerprint (AFP, 1800-950 cm-1). In both spectral regions, data was processed using a Savitzky-Golay filter (2nd derivative order; 11 points window in the HW region, 17 points window in the AFP region) followed by unit vector normalization. Data processing was performed with the Quasar software (version 1.7). 3. Instrument- or software- specific information needed to interpret the data: Any software able to read csv files. 4. Instruments, calibration and standards information: Hyperion 3000 microscope, Vertex 70 Spectrometer (Bruker Optics GmbH). 5. Environmental or experimental conditions: SR-FTIRM measurements were carried out on dried cells at room temperature. Background spectra were collected every 10 cells to compensate for varying ambient conditions inside the MIRAS beamline.