Here we have surveyed the miRNA complement from a number of adult tissues in the freshwater pulmonate gastropod Lymnaea stagnalis. In addition we focus on miRNAs involved in biomineralization by inducing shell regeneration. Specifically, we compare the miRNA complements of shell-regenerating mantle regions against immediately adjacent mantle regions. This approach allows us to identify miRNAs that potentially regulate either inhibitors or enhancers of shell formation.