Series of differential interference contrast microscopy (DIC) videos of primary keratocytes from Atlantic salmon scales acquired at room temperature (RT). The salmon from Kårvika (Tromsø Aquaculture Research Station) was about 1 kg +- 0.4 kg of either sex or smolt (about 10 to 15 cm long). The triploid salmon was > 1 kg and from a fish farm at Senja. The fish and cells were untreated. Unless specifically mentioned, the videos were acquired as single panels (field-of-view (FOV) 668 µm^2) 5 seconds apart for up to 20 hours, or larger areas (multiple panels (typically 4 by 4) of a combined FOV 2.7 mm^2) 2 minutes apart for up to 24 hours. The aim of the study was to investigate salmon skin cell dynamics, which are important to the innate immunity of fish.

This is the first dataset in a planned series of microscopy time-lapse data of fish cells.