Protein density profile near protein-resistant self-assembled monolayers: Sensitivity enhancement using a magnetic reference layer

DOI

Oligo(ethylene glycol) self-assembled monolayers (SAMs) effectively prevent the adsorption of proteins from biological media. Despite the importance of this effect for bio-technical applications, the molecular-level understanding of the mechanisms leading to protein resistance is limited. Various mechanisms have been proposed to explain the prevention of irreversible adsorption. However, the small length scales of the interactions make studies, especially in-situ, rather difficult. This proposal aims at obtaining high resolution structural information of the interfacial region at the SAM/protein solution interface with the aid of polarised neutron reflectivity using a magnetic reference layer.This will allow the determination of the protein density profile for different protein sizes and a study of the transition from a resistant to an adsorbing surface using temperature switchable SAMs.

Identifier
DOI https://doi.org/10.5286/ISIS.E.24068347
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/24068347
Provenance
Creator Dr Frank Schreiber; Dr Maxmilian Skoda
Publisher ISIS Neutron and Muon Source
Publication Year 2012
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Photon- and Neutron Geosciences
Temporal Coverage Begin 2009-05-29T07:50:23Z
Temporal Coverage End 2009-06-01T06:12:01Z