Repeat of “Assembling floating, very sparsely tethered, protein-lipid bilayers”.

DOI

This was granted 2 days but problems with our samples meant that no data was got. After successful practice on Biacore offline, failure was due to using a new (faulty) batch of thiolipid /linker. By using a single batch for both we will avoid this problem in future. Tethered floating lipid bilayers are useful models of biological membranes in which a phospholipid bilayer is tethered to a solid surface by a molecular linker. They promise an additional method to help us build accurate models of all types of membranes. Our previous POLREF experiments showed that we can assemble lipid only bilayers at very low tether densities which is important to minimise their effect on the membrane. Here we wish to show that we can insert and characterise proteins within the lipid bilayer which is another important step in creating useful models of living membranes in neutron tractable sample environmen

Identifier
DOI https://doi.org/10.5286/ISIS.E.87864464
Metadata Access https://icatisis.esc.rl.ac.uk/oaipmh/request?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:icatisis.esc.rl.ac.uk:inv/87864464
Provenance
Creator Dr Andrew Caruana; Dr Martynas Gavutis; Dr Nico Paracini; Dr Luke Clifton; Professor Jeremy Lakey
Publisher ISIS Neutron and Muon Source
Publication Year 2020
Rights CC-BY Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Contact isisdata(at)stfc.ac.uk
Representation
Resource Type Dataset
Discipline Biology; Biomaterials; Engineering Sciences; Life Sciences; Materials Science; Materials Science and Engineering
Temporal Coverage Begin 2017-11-30T08:30:00Z
Temporal Coverage End 2017-12-02T08:30:00Z