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Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribe...
combined samples sequenced with primers modified for forward barcoding on the 515f site, extended to include V5 and improved ITS -
DNA extraction comparison testing 2019-2020
Our DNA extraction protocol is years old, and we want to compare new extraction methods to address outstanding issues. For more details, check out the motivation and... -
Global patterns of 16S rRNA diversity at a depth of millions of sequences per...
The ongoing revolution in high-throughput sequencing continues to democratize the ability of small groups of investigators to map the microbial component of the biosphere. In... -
Knight skin biogeography comparison
Knight_skin_biogeography - Comparison of skin samples from a rat (mammal), cyclostomid (fish), iguana (reptile), bull frog (amphibian) and a bird (pigeon) to determine if the... -
Earth Microbiome Project Multi-omics (EMP500)
The Earth Microbiome Project Multi-omics component (EMP500) involves amplicon and shotgun metagenomic sequencing and metabolomic profiling of over five hundred microbial... -
Knight_extraction_test
One plate of samples was extracted using several different extraction tools (new, faster) for comparison with the existing pipeline -
Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation. However,...