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Conformational change of glycerol faciliitator protein on substrate binding
A large family of membrane channel proteins selective for transport of water (aquaporins) or water plus glycerol (aquaglyceroporins) has been found in diverse life forms. E.... -
Oil disperse phospholipid stabilised microemulsions prepared using non-aqueou...
It is the aim of the proposal is to perform small angle neutron scattering (SANS) studies combined with contrast variation to determine the size and structure of the... -
Effect of reversing lipid head group charge on vesicular properties
Zwitterionic lipids such as phosphatidylcholine (PC) are major components of the membranes that compartmentalize organisms. Although there is a large literature on PC, cationic... -
Interaction of the nucleic acid, DNA, with a model biomembrane at the oil-wat...
The information gained in the study will be invaluable in aiding our understanding of the structure and transport properties of the cell membrane as well as helping in the... -
Follwing in situ oxidation of lipids rafts by SANS
The lateral organisation of lipids in the cell membrane is crucial for many biological processes, however techniques to follow this organization are scarce. We have developed a... -
Solubilisation of steroidal drug in dodecyl sulphate micelles: effect of anion
An number of potential drugs are extremely water insoluble, limiting their commercialisation as medicines. Solubilisation of drug in surfactant micelles is a means of increasing... -
Microdomains in model membranes using contrast variation SANS
In the present proposal small angle neutron scattering will be used in combination with contrast variation as a non-invasive method to detect the presence and size formation of... -
Nanosuspsomicroemulsions: nanosuspension stabilty
At Kings College London we have recently developed a novel combination formulation, a nanosuspsomicroemulsion (NSME), comprising of a microemulsion (ME) containing one drug and... -
Probing the kinetics of the formation of lipid, peptide and DNA/siRNA complex...
Complexes prepared from cationic, dichain lipid (L) vesicles, positively chargely targeting peptides (P) and negatively charged DNA (D) or siRNA (R), known as LPDs and LPRs... -
Effect of serum on the structure of gene delivery vehicles
To date complexes prepared from vesicles composed of dichain, cationic lipids (L) with negatively charged DNA (D), known as LD complexes have emerged as one of the major... -
Effect of oxidation on lipid raft formation: an off-specular neutron reflecti...
The very heterogeneous, but non-random, arrangement of lipids and proteins in cell membranes, known as rafts are of major scientific interest. Lipids in cell membranes are... -
Optimizing micellar drug solubilisation using surfactant designed with a hydr...
In our recent research at Kings we have shown that significant increases in micellar drug solubilization can be achieved by matching' the hydrophobe of a drug with the... -
Determination of the absorbed amount and location of a hydrophobic drug in su...
Many drugs are both poorly water-soluble (hydrophobic) and lipophilic in nature and are therefore incorporated into surfactant micelles and monolayers. As the incorporation of... -
Evolution of raft formation over time using contrast variation SANS
Small angle neutron scattering will be used in combination with contrast variation as a non-invasive method to detect the presence and size formation of lipid microdomains or... -
Ibuprofen containing surfactant monolayers: location and stiochiometry of ibu...
An increasing number of potential drug molecules are extremely water insoluble, limiting their commercialisation as medicines. Solubilisation of drug in surfactant micelles is a... -
Rhamnolipids and Amphotericin B: a potential highly specific delivery system ...
Rhamnolipids are a type of glycolipid biosurfactant, produced as secondary metabolites by various Pseudomonas and Burkholderia species; they have been shown to possess... -
Effect of Temperature on the Transbilayer Structure of a Model Erythrocyre Me...
In recent years, it has become understood that rather than being homogenous mixtures, biomembranes show a complex phase behaviour, with regions of uniform phases, and others... -
Molecular architecture of vesicles formed by novel sterol-modified phospholipids
A family of sterol-modified glycerophospholipids (SML) have been synthesized in which the sn-1 or sn-2 position is covalently attached to cholesterol and the alternative... -
Impact of homo- and hetero-association of complex molecular nano-sized drug d...
The present proposal concerns a PEGylated, hydrophobic drug containing polylysine dendrimer that demonstrates unexplained batch-to-batch variability in that two batches of the... -
Structural studies of synthetic cutin using neutron reflectivity
The aerial parts of higher plants are covered in a waxy layer known as the cuticle. This layer - which comprises an insoluble bio-polyester (cutin) framework impregnated with...
